RESPONSIBILITIES
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Clone, express, and purify therapeutic antibodies
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Characterize antibody candidates for cell binding using FACS and high-content screening (HCS) instruments (Cell Insight CX5 Platform)
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Characterize antibody candidates using analytical methods (e.g. SDS-PAGE, SEC, IEF, DSC, DSF, HIC, Nano DSF/DLS)
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Develop and perform protein-protein interaction assays, including kinetic, epitope binning, blocking assays (ELISA, SPR, BLI)
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Implement new technologies as required to improve efficiency of the group (e.g. AC-SINS, automation of production or DNA preps using TECAN)
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Be a hands-on presence in the laboratory
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Perform data analysis and documentation
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Write formal technical reports, methods and procedures
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Accomplish goals under project team timelines
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Communicate effectively and in a timely manner protein production status and research progress to senior management
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Present results during internal meetings
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Support a culture that embraces scientific excellence, continuous learning, and increasing team’s ability to deliver novel drug candidates
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Comply with HSE guidelines
EDUCATION AND DESIRED EXPERIENCE
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MSc or BSc degree in Biochemistry, Structural Biology, Biotechnology or a related discipline is required. Industry experience in antibody characterization is preferred
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Proficiency in molecular biology techniques (PCR, cloning, primer design) and DNA sequence analysis software is required.
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Hands-on experience in protein production, purification and characterization (practical knowledge of HPLC and/or AKTA protein purification systems is preferred)
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Experience with protein-protein interaction methods (SPR, BLI or related technologies), hands-on experience with Biacore and/or Octet systems is desirable
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Hands-on experience in cell culture is preferred.
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Knowledge in Design of Experiment and statistical analysis is a plus.
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Scientific curiosity, problem solving abilities, capacity to plan experiments and analyze results.
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Self-motivated team player with strong organization skills.
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Able to work with flexibility in a changing and demanding research environment
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Professional level in English, both written and verbal.
LOCATION:
You will join us, a biotech community at the Biopole